FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004982950

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004982950
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12121693
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATTGA26800.0115.027571
TAAACCG36300.0108.932811
CCGAACG28900.0106.876519
TACGGGC45050.0105.2020341
ACGATAC44750.0103.492991
CCGGTAG50000.0102.2777941
TACCTTA44850.0102.138081
CGCTATC29450.0101.9867551
CCTTACG34750.0101.769981
TCCCGAT41950.0100.442371
CGTCTAC37400.099.758791
GACCAAT38700.099.0133361
CACCGAA22750.098.4237758
TTAGGCA45250.098.370051
AGTACGA35150.098.3188868
TGACTAG36100.097.764661
ACTAGTC34250.097.752053
TGACGGC32300.097.670761
TACGGTA43450.097.6379241
TACTTAC60300.096.632721
Produced by FastQC (version 0.11.5)