FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004982952

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004982952
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12265468
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATTGA24250.0113.768181
TAAACCG34350.0110.094681
CCGAACG30000.0108.956739
CGCTATC31650.0108.789751
CCGGTAG47750.0107.8614961
TACGGGC45700.0103.557831
CACCGAA23950.0100.406198
CGTCTAC36400.099.936421
ACTAGTC31100.099.546873
TCCCGAT40500.099.245991
ACGATAC48700.098.657621
TTAGGCA42550.098.188861
AGTACGA35200.097.769818
CCTTACG33350.097.412461
GACCAAT38850.097.34241
TACTTAC61000.097.0679241
TGACGGC30900.096.7438661
TACACGA33450.096.259851
TACCTTA44500.095.8286741
GCGCGAT35100.095.223621
Produced by FastQC (version 0.11.5)