FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004982954

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004982954
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11817595
Sequences flagged as poor quality0
Sequence length150
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATTGA25650.0112.325361
TAAACCG34950.0110.258431
CCGGTAG46850.0107.3126751
CGCTATC28700.0104.152911
ACGATAC43600.0103.417271
CCGAACG30100.0103.333359
CCTTACG29250.0102.933241
TCCCGAT41300.0102.723651
TACGGGC46000.0102.5625151
TACCTTA44250.0101.735371
CACCGAA24100.099.483368
TTAGGCA43800.099.327161
TACTTAC59000.098.8867651
GCGCAAC25400.097.55061
TGACTAG32400.096.927441
CGTCTAC35350.096.78531
ATTCTAC69150.096.6631551
AGTACGA33100.096.360498
ACGAGCC27050.096.126941
TTGAACG29700.095.06811
Produced by FastQC (version 0.11.5)