FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004982955

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004982955
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11817595
Sequences flagged as poor quality0
Sequence length150
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCC4453003.7681101780861503No Hit
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTC3062102.591136352193488No Hit
CTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCC2088661.7674154512826001No Hit
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTC1413961.1964871024942048No Hit
GATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTC476190.40295000801770575No Hit
TGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCT407630.34493481964816025No Hit
GATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTC223120.18880322095993304No Hit
TGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCT193840.1640266060903255No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGAATG184780.1563600715712461No Hit
CAGGCAGTATCTGGAGTCATTGAGGGCGGGCTGCTCCTTGAGGGGCTGCG166420.1408239155259594No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGTTTT154100.13039878249339226No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATTTGGGATG119260.10091731862532098No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATTTGGAATG118670.10041806306613148No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGATG597000.0114.921591
GTCTCTC521950.0100.333222
AGTCTCT530900.098.8588261
TCTCTCA552000.095.167033
TCAGCTG577750.090.562547
CAGCTGG598000.087.531228
AGCTGGT612900.085.2623149
TCTCAGC730500.071.912665
CTGATGG1395950.071.243231
GGCTCAA1426100.069.648616
CTCAAAC1434950.069.21738
GCTCAAA1435700.069.20187
TCAAACA1450400.068.534599
CTCAGCT769700.068.315716
CTCTCAG777900.067.7436754
ATGGCTC1484500.067.044464
GATGGCT1506950.066.031323
TGGCTCA1533750.064.7601555
TGATGGC1673350.059.7082982
TGATACG15900.049.8122673
Produced by FastQC (version 0.11.5)