FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004982956

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004982956
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11948757
Sequences flagged as poor quality0
Sequence length150
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATTGA26800.0113.432911
TAAACCG33750.0108.643911
CCGGTAG46150.0107.393531
TACGGGC45050.0106.977571
CCGAACG31400.0106.621939
CGCTATC30600.0104.2902451
ACGATAC46450.0102.667661
TTAGGCA45850.0102.597131
CACCGAA23450.0101.31988
CGTCTAC37900.0100.929181
TACACGA32900.099.626951
TACGGTA43200.099.552451
TCCCGAT40400.098.428091
ACTAGTC31550.098.1307453
TGACGGC31700.097.944351
TACCTTA44800.097.444211
GACCAAT42200.097.1317441
ACGAGCC27350.096.665191
TGACTAG32050.096.2004551
TCGGGAC56050.096.136291
Produced by FastQC (version 0.11.5)