FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004982975

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004982975
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8046222
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCC2750173.417964356439581No Hit
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTC1796792.2330852914572827No Hit
CTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCC1283721.5954319928035792No Hit
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTC828611.0298125008233678No Hit
GATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTC342590.42577746425589547No Hit
TGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCT273620.3400602170807616No Hit
GATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTC158370.19682529266530305No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATATGGGTTT149130.18534164232605066No Hit
TGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCT129880.1614173707859415No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGTTTT119980.1491134597081711No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATTGGGTATG110750.13764223756192656No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGAATG106930.1328946678329283No Hit
CAGGCAGTATCTGGAGTCATTGAGGGCGGGCTGCTCCTTGAGGGGCTGCG98300.12216913726715471No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCAGGCCAG97310.12093874615937766No Hit
GCTCAGTGTCACTTGCTGTCCTCTCGTTTTGATCAGATATCTTGGAGTTT86100.10700674179757903No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATAGGGCTGG84730.10530407935550373No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGATG353900.0112.982751
AGTCTCT456100.0111.303971
TCTCTCA505450.0100.683673
GTCTCTC523450.097.2156752
AGCTGGT565400.090.005829
CAGCTGG577050.088.46328
TCAGCTG609750.083.6009757
CTCAGCT661600.077.1925056
CTCTCAG671500.075.947234
TCTCAGC684800.074.4511645
CTGATGG816800.073.825871
GGCTCAA877650.068.543426
CTCAAAC881900.068.1950768
GCTCAAA886950.067.814927
TCAAACA893900.067.3037649
GATGGCT896300.067.1734163
ATGGCTC912450.066.031824
TGGCTCA934150.064.382315
TGATGGC985750.061.464162
CTTCCGA16500.041.018954
Produced by FastQC (version 0.11.5)