FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004982976

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004982976
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8140850
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATTGA25550.0113.903091
GGCTCGA23950.0111.887731
TACGGAT30550.0110.587671
TTGAACG27600.0109.618861
ACGAGCC26550.0108.527681
GCGCAAC27300.0106.073881
TGACTAG27750.0105.3921051
CGGACTG24550.0104.165021
CATCGAA21600.0103.717421
CTCTACG31900.0102.9720151
GTCGAAA8700.0102.6173258
GTACGTA24400.0101.853121
ACTAACG11750.0101.745822
GGTATTG29950.099.57461
GACACGC32850.098.8977051
AGCGTAT32100.097.842261
TTCGAAG28600.097.726141
CGCTATC21600.097.7144851
GATCTAG25400.096.708741
GACCAAT29550.096.5345461
Produced by FastQC (version 0.11.5)