FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004982977

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004982977
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8140850
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCC2766023.3977041709403806No Hit
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTC1820352.236068715183304No Hit
CTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCC1287031.5809528489039844No Hit
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTC835571.0263915930154714No Hit
GATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTC345590.4245134107617755No Hit
TGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCT278580.3422001388061443No Hit
GATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTC160740.19744866936499259No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATATGGGTTT148200.18204487246417758No Hit
TGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCT128860.15828813944489825No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGTTTT122130.15002118943353582No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATTGGGTATG111010.1363616821339295No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGAATG106130.1303672220959728No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCAGGCCAG98330.12078591301891081No Hit
CAGGCAGTATCTGGAGTCATTGAGGGCGGGCTGCTCCTTGAGGGGCTGCG98320.12077362928932484No Hit
GCTCAGTGTCACTTGCTGTCCTCTCGTTTTGATCAGATATCTTGGAGTTT86590.10636481448497394No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATAGGGCTGG85390.10489076693465671No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGATG350100.0113.8814851
AGTCTCT460600.0111.265661
TCTCTCA507000.0101.011613
GTCTCTC523600.097.80752
AGCTGGT572050.088.958279
CAGCTGG583750.087.532978
TCAGCTG615700.083.002397
CTCAGCT671500.076.373626
CTCTCAG679650.075.447194
TCTCAGC687700.074.511685
CTGATGG823800.073.409371
CTCAAAC880800.068.647128
GGCTCAA880500.068.564636
GCTCAAA887450.068.100277
TCAAACA894700.067.709389
GATGGCT903850.066.968583
ATGGCTC914200.066.171034
TGGCTCA933800.064.658775
TGATGGC987800.061.4755942
TACGGCG9150.052.7205733
Produced by FastQC (version 0.11.5)