FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004982978

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004982978
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8555207
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCTCGA30000.0111.6451341
CGATTGA31750.0111.1630551
TACGGAT32700.0109.255171
TTGAACG28200.0107.788261
GCGCAAC29850.0107.380091
TGACTAG28850.0106.6080861
CTCTACG32950.0105.584421
ACGAGCC28650.0102.324091
CGCTATC25650.0101.655131
CGGACTG24550.0101.222181
GTCGAAA8900.099.50288
CATCGAA23700.099.0780261
ATGCGAT24050.096.7376561
ACGATGT34700.096.730741
AGCGTAT32800.096.185231
TGACGGC23400.096.038821
GACACGC31850.095.661911
TCGGGAC31200.095.115371
GTACGTA26350.094.854281
CGAATGT38950.094.6826861
Produced by FastQC (version 0.11.5)