FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004982979

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004982979
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8555207
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCC2882603.3694099979112138No Hit
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTC1880462.1980298080455563No Hit
CTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCC1365401.5959870988510272No Hit
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTC880891.0296536366682887No Hit
GATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTC362170.4233328311050802No Hit
TGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCT287250.3357604322139721No Hit
GATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTC167320.19557679901842234No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATATGGGTTT158500.1852672880971787No Hit
TGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCT134240.15691028866981244No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGTTTT127990.14960479623695838No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATTGGGTATG114960.13437430561294425No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGAATG114460.13378986621831593No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCAGGCCAG102960.12034776014186448No Hit
CAGGCAGTATCTGGAGTCATTGAGGGCGGGCTGCTCCTTGAGGGGCTGCG102180.11943603468624431No Hit
GCTCAGTGTCACTTGCTGTCCTCTCGTTTTGATCAGATATCTTGGAGTTT93550.10934861073495941No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATAGGGCTGG90670.10598223982190026No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGATG369100.0113.028331
AGTCTCT494050.0110.403341
TCTCTCA541900.0100.6340943
GTCTCTC558550.097.615562
AGCTGGT605200.089.9045949
CAGCTGG617900.088.313098
TCAGCTG657000.083.0901957
CTCTCAG709250.077.0820164
CTCAGCT711750.076.861846
TCTCAGC719200.076.045635
CTGATGG862150.074.509191
GGCTCAA925300.069.363186
CTCAAAC925500.069.323648
GCTCAAA933500.068.76047
TCAAACA939950.068.372829
GATGGCT942350.068.054713
ATGGCTC960550.066.795234
TGGCTCA985800.064.923675
TGATGGC1036450.062.2430082
TACGGCG9600.048.0007173
Produced by FastQC (version 0.11.5)