FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004982980

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004982980
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8654021
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCTCGA27450.0115.4678651
TACGGAT31600.0113.981321
CGATTGA27850.0113.292121
TGACTAG27200.0109.908171
GCGCAAC29650.0108.6009451
CTCTACG34850.0108.519391
TTGAACG31550.0107.3122561
ACGAGCC26400.0105.0527951
ACTAGTC14000.0102.342843
CATCGAA24400.0101.854461
GTCGAAA9200.0101.737348
CGGACTG25400.0100.964131
TACCTTA28600.098.231181
GTACGTA29450.098.086641
CGCTATC25450.097.086111
TGACGGC25550.096.988071
GACCAAT31700.096.805741
ATGCGAT24050.096.447641
GACACGC33000.096.048271
GATCTAG26400.095.502541
Produced by FastQC (version 0.11.5)