FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004982981

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004982981
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8654021
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCC2904703.3564744065215466No Hit
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTC1905282.201612406533333No Hit
CTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCC1373141.5867074970120827No Hit
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTC880041.0169145649172795No Hit
GATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTC364990.42175770084218656No Hit
TGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCT288300.33313993575934236No Hit
GATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTC169390.19573560082648284No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATATGGGTTT159220.18398383826431666No Hit
TGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCT136950.15825013597725265No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGTTTT129930.15013829987239458No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATTGGGTATG119200.1377394392733736No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGAATG115740.13374129783137803No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCAGGCCAG105090.12143487980905061No Hit
CAGGCAGTATCTGGAGTCATTGAGGGCGGGCTGCTCCTTGAGGGGCTGCG104560.12082244773845591No Hit
GCTCAGTGTCACTTGCTGTCCTCTCGTTTTGATCAGATATCTTGGAGTTT93340.10785737635718702No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATAGGGCTGG91240.10543075871898161No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGATG374700.0112.824691
AGTCTCT493850.0110.1619641
TCTCTCA537000.0101.4294053
GTCTCTC555000.098.022592
AGCTGGT603150.090.090359
CAGCTGG614600.088.704328
TCAGCTG644850.084.5101857
CTCAGCT705600.077.550446
CTCTCAG713550.076.454344
TCTCAGC724600.075.208945
CTGATGG879950.074.912651
GGCTCAA943600.069.862986
CTCAAAC945900.069.69278
GCTCAAA951950.069.227497
TCAAACA959800.068.7138069
GATGGCT966350.068.1735463
ATGGCTC982050.067.1569754
TGGCTCA1003700.065.600795
TGATGGC1058800.062.5227782
ATACGGC11300.045.2385985
Produced by FastQC (version 0.11.5)