FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004982983

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004982983
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10042384
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCC3464303.44967888103064No Hit
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTC2234282.2248501949337927No Hit
CTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCC1619951.6131129819373566No Hit
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTC1031981.0276245162503246No Hit
GATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTC432490.43066467085903104No Hit
TGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCT342350.34090510779113803No Hit
GATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTC201280.200430495388346No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATATGGGTTT187190.18639996239936651No Hit
TGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCT161920.1612366147321194No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGTTTT150350.14971544605344708No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATTGGGTATG138230.13764659865625534No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGAATG131870.1313134411111943No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCAGGCCAG124040.12351648771845411No Hit
CAGGCAGTATCTGGAGTCATTGAGGGCGGGCTGCTCCTTGAGGGGCTGCG121970.12145522417784463No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATAGGGCTGG106260.10581152841795335No Hit
GCTCAGTGTCACTTGCTGTCCTCTCGTTTTGATCAGATATCTTGGAGTTT105050.10460663523721062No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGATG441450.0112.7785341
AGTCTCT563700.0110.9463961
TCTCTCA622700.0100.516433
GTCTCTC646300.096.788762
AGCTGGT698800.089.35069
CAGCTGG711550.088.063248
TCAGCTG751400.083.239967
CTCAGCT822950.076.43386
CTCTCAG825300.076.050394
CTGATGG1033500.075.042331
TCTCAGC839250.074.709075
GGCTCAA1117500.069.3734746
CTCAAAC1119400.069.304768
GCTCAAA1128300.068.732917
GATGGCT1134650.068.4264453
TCAAACA1138300.068.2236259
ATGGCTC1157500.067.0569844
TGGCTCA1183700.065.41465
TGATGGC1240550.062.8075872
TACGGCG10850.051.0983926
Produced by FastQC (version 0.11.5)