FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004982984

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004982984
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10154882
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATTGA31250.0114.106041
GGCTCGA30600.0108.996621
TACGGAT36950.0108.786061
GCGCAAC35450.0107.9025651
TTGAACG38450.0105.478881
CTCTACG39700.0105.0611
ACGAGCC33850.0104.915991
ACTAACG14500.0103.3093642
CGGACTG29250.0102.698391
TGACTAG34750.0102.4060441
GTCGAAA10200.0102.349578
GACCAAT38000.0102.178291
GACACGC36800.0101.00791
TATTACC34100.099.499291
CATCGAA30150.099.1549151
TTCGAAG41500.097.553231
CACGAAA36700.096.716158
CGCTATC28900.096.713551
GTCCGAA28950.096.245538
ACTAGTC17400.095.585413
Produced by FastQC (version 0.11.5)