FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004982987

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004982987
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9209519
Sequences flagged as poor quality0
Sequence length150
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCC3165883.4376171003067584No Hit
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTC2097672.277719390122329No Hit
CTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCC1464521.5902242017199812No Hit
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTC955881.0379260849562284No Hit
GATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTC398920.43316051576635No Hit
TGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCT319120.3465110392844621No Hit
GATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTC183150.19887032102328034No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATATGGGTTT169510.18405955837650154No Hit
TGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCT147410.16006264822299623No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGTTTT136220.14791217652083674No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATTGGGTATG125760.13655436293686998No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGAATG121980.13244991405088583No Hit
CAGGCAGTATCTGGAGTCATTGAGGGCGGGCTGCTCCTTGAGGGGCTGCG114570.12440389123471052No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCAGGCCAG109720.1191376009974028No Hit
GCTCAGTGTCACTTGCTGTCCTCTCGTTTTGATCAGATATCTTGGAGTTT95920.10415310506444472No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATAGGGCTGG94260.1023506222203353No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGATG410850.0112.7365951
AGTCTCT522750.0110.658781
TCTCTCA572650.0101.1913
GTCTCTC592100.097.733242
AGCTGGT639600.090.224439
CAGCTGG650400.088.947648
TCAGCTG685100.084.528857
CTCAGCT751550.077.294976
CTCTCAG767400.075.5859154
TCTCAGC772050.075.167975
CTGATGG949850.074.168451
CTCAAAC1025600.068.650788
GGCTCAA1027050.068.5701146
GCTCAAA1034250.068.134167
TCAAACA1041900.067.687349
GATGGCT1043350.067.485063
ATGGCTC1059600.066.524864
TGGCTCA1090800.064.490045
TGATGGC1135250.062.385022
TACGGCG12750.053.648386
Produced by FastQC (version 0.11.5)