FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004982988

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004982988
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9316513
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATTGA30100.0114.878931
TACGGAT34000.0109.1173861
GGCTCGA31150.0108.925241
TGACTAG30900.0108.873961
ACGAGCC31750.0108.6819461
TTGAACG33600.0107.414791
CTCTACG37800.0105.199321
GCGCAAC31550.0104.8042761
TGACGGC26550.0103.920171
GTCGAAA9050.0101.832618
GACCAAT34850.099.2211151
TTCGAAG34150.098.93451
GTACGTA30400.098.579231
GGTATTG33050.098.085921
TACCTTA29450.097.600781
CGCTATC25450.097.372491
ACTAACG14200.096.880952
GTCCGAA26350.096.72718
GATCTAG28650.096.5544361
CATCGAA26100.096.051551
Produced by FastQC (version 0.11.5)