FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004982989

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004982989
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9316513
Sequences flagged as poor quality0
Sequence length150
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCC3208293.4436596610770573No Hit
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTC2104032.2583878753778373No Hit
CTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCC1479271.5877936305139058No Hit
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTC970271.0414518822653926No Hit
GATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTC406580.43640791356165126No Hit
TGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCT322840.3465244990266208No Hit
GATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTC186790.20049346788868325No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATATGGGTTT169920.1823858347001716No Hit
TGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCT149040.15997401602938782No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGTTTT137210.14727613217520333No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATTGGGTATG125800.13502906076554608No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGAATG124480.1336122216541747No Hit
CAGGCAGTATCTGGAGTCATTGAGGGCGGGCTGCTCCTTGAGGGGCTGCG114380.12277125572625724No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCAGGCCAG112340.12058159528141055No Hit
GCTCAGTGTCACTTGCTGTCCTCTCGTTTTGATCAGATATCTTGGAGTTT99000.10626293335285422No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATAGGGCTGG95530.10253836387068853No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGATG412100.0113.226941
AGTCTCT521750.0110.757421
TCTCTCA571800.0101.113363
GTCTCTC595700.097.0437852
AGCTGGT642250.089.717379
CAGCTGG656600.088.184728
TCAGCTG687250.084.1470957
CTCAGCT758500.076.519216
CTCTCAG765100.075.689754
TCTCAGC772450.075.072075
CTGATGG961950.073.834081
GGCTCAA1027400.069.0784156
CTCAAAC1029100.068.977188
GCTCAAA1034350.068.606197
TCAAACA1043200.068.1273359
GATGGCT1048200.067.735133
ATGGCTC1064450.066.6875464
TGGCTCA1087600.065.175395
TGATGGC1147700.062.116222
TACGGCG11400.042.947833
Produced by FastQC (version 0.11.5)