FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004996083

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004996083
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15662549
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGGGG810700.030.624235145
CTGTCTA104400.030.07338145
CGGGGGG97450.015.973717145
GTTCTAG61500.015.2511541
GTGCTAG52450.014.8517081
GCGAATA32400.014.657615145
GTACTAG28000.014.5726071
GTACTAA44050.014.5560671
GTCCTAG51650.014.1583731
GCCGTAT16902.935136E-514.050494145
GGTCTAG41250.014.0025491
GTGTAGG71200.013.8432331
CTGTACT103050.013.4735764
GTCCTAA46750.013.3753441
GTATAGG36250.013.3026441
GCTCTAG62150.013.0453241
GTCTAGG50100.012.7982981
GTATACG10150.012.5299631
GTATAGA51900.012.4565291
CTATACT50400.012.4074074
Produced by FastQC (version 0.11.5)