FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004996090

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004996090
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15228175
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG26150.018.3266941
AGGGGGG119050.017.87274145
GCCGTAT63200.017.036276145
CCGTATA15257.8571793E-716.810188145
CTGTCTA99950.016.414951145
GTTCTAG61500.016.1912291
GCGTATA12006.183219E-414.954064145
GTGCTAG49750.014.8776741
GTCCTAG54300.014.8077971
GTACTAA43700.014.6222131
GCCGTAA19656.939172E-714.350683145
GCTCTAG63150.014.0817431
GTATTAG43950.014.0544031
GTATTAA72800.014.0437531
TGACGCG20108.9599234E-714.0294145
GTATACG11050.013.974911
CGTATCA64500.013.910759145
TACGCGA5003.2889147E-813.8307352
GTATAGG37850.013.3650641
GTCCTAA46350.012.9820131
Produced by FastQC (version 0.11.5)