FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004996116

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004996116
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16873302
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGGGG1418000.050.13261145
CGCCGTA83200.038.078297145
GCCGTAA18201.8189894E-1236.646782145
AGGGGGG113800.036.630684145
CGTATCA83000.036.161102145
CCGTATA14050.035.60346145
GCCGTAT83300.032.02744145
TCGCCGT94700.028.171974145
CGGGGGG124500.027.67887145
CTGTCTA96900.025.811588145
AAGGGGG118350.025.360134145
ATATCGG24450.025.005745145
GTCGCCG107450.024.82909145
TAGATCG16251.6720767E-523.942564145
CGAATAA29203.7471182E-1022.841486145
TCGGTGG166300.022.72703145
CATTAAA186300.021.480562145
GACGAAA39251.8189894E-1221.241129145
AAAAAAA1052250.021.075602145
TGGGGGG151950.020.849716145
Produced by FastQC (version 0.11.5)