FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004996120

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004996120
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16670119
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTATA15900.054.652878145
GGGGGGG1408350.047.78052145
AGGGGGG114650.037.423397145
TGACGCG23700.036.665855145
CGTAACA19001.8189894E-1234.30187145
CGTATCA84100.032.93551145
CGCCGTA84750.032.04206145
CTGTCTA102100.030.852644145
CCTGTCG50800.028.866241145
CGGGGGG118950.026.482178145
GACGAAA40100.025.733532145
CGCCGTT17351.1726643E-625.042671145
AAAAAAA1013750.024.376463145
TCGCCGT97050.024.063738145
GCCGTAT84000.023.922832145
GTATCAT127950.023.346004145
GTGGTCG131450.023.137564145
GTCGCCG106250.023.002434145
GCCGTAA19306.475602E-1022.512455145
TGGGGGG149400.022.175295145
Produced by FastQC (version 0.11.5)