FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004996134

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004996134
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17217553
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGTCTA116950.024.61929145
CCGTATA16950.023.812967145
TGACGCG21500.020.025043145
GTCCTAG59250.017.0542281
GTTCTAG76750.017.029471
GTACTAG31650.016.1365911
CGCCGTA55050.016.13053145
AGGGGGG125200.015.904474145
CCTGTCG48300.015.599218145
GACGAAA38350.015.436514145
CGTATCA61300.014.485898145
GCCGTAA18804.191719E-614.313113145
GGGGGGG2137450.014.162781145
GTACTAA50000.014.0586161
GTCTTAG59600.013.91341
GTACAGG104250.013.8015481
TCGCCGT68500.013.748947145
GCTCTAG71700.013.1738241
GTGCTAG60000.013.0884231
CGGGGGG142250.013.052353145
Produced by FastQC (version 0.11.5)