FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004996135

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004996135
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16967496
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGGGG725700.032.138973145
CTGTCTA116900.029.037333145
GTACTAG32400.014.0429661
GTACAGG112700.013.9599461
GTATTAG56950.013.5722281
GTCCTAG57050.013.548441
GTGCTAG59500.012.898431
GTATAGG43100.012.8460581
TATACTG78250.012.6792375
GTGTAGG80300.012.4245771
GTTCTAG70800.012.3108961
GTACAGA116950.012.2808051
GTCTAGC42250.012.1962671
GTCTTAG57200.012.1712031
CGTATTA15850.00307188612.089778145
CTGTACT119300.011.9462554
GTACTAA49600.011.9362491
GTTCTAA78100.011.8620971
CTATACT61650.011.8254444
GTGCAGG208200.011.6903641
Produced by FastQC (version 0.11.5)