FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004996136

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004996136
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16967496
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGTCTA113700.024.885942145
CCGTATA16650.024.048376145
GTCCTAG62350.017.0352291
GTACTAG32050.016.1408711
CCTGTCG47450.014.626682145
GTATAGG44100.014.2263471
GTTCTAG72150.014.1874291
GTGCTAG60950.014.175981
GTCTTAG58950.013.9100791
CGCCGTA56550.013.6890745145
GGTCTAA50400.013.6491811
GCTCTAG70400.013.4456841
GTCCTAA54250.013.390641
GCCGTAA18004.759076E-513.346848145
GCCGTAT58800.013.165257145
GTATTAG53100.013.1624381
TGACGCG20301.0067932E-513.149603145
TCGCCGT68200.012.916305145
GCCCTAG58300.012.8380031
TACACAA135150.012.640743145
Produced by FastQC (version 0.11.5)