FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004996137

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004996137
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17553937
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGTCTA123000.039.797882145
GGGGGGG775550.032.088074145
CGGGGGG103200.018.28443145
GTCCTAG63600.018.016651
GGCGTAT16308.0199425E-716.777391145
GTACTAG32350.015.0834481
GTATTAG58350.014.5638331
TACGCGA4651.408207E-714.1489942
AGGGGGG98450.013.6110735145
GGTCTAG47150.013.4884241
GTGCTAG61250.013.33721351
GTATAGG43800.013.1431681
GTCTAGG57900.012.8779061
GTATTAA93800.012.6835781
GTTCTAG73350.012.6319821
GTCCTAA57000.012.3117561
TCTAGAC59750.012.2961473
GTACAGG111200.012.2273341
GTACAGA127100.012.2074731
TGTACTG112150.012.1246225
Produced by FastQC (version 0.11.5)