FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004996164

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004996164
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15507867
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGGGG607100.047.21752145
CTGTCTA97750.030.422197145
CGGACTA13751.1197699E-825.148159145
AGGGGGG73800.022.95877145
TATGCCG85600.018.98598145
CGGGGGG62250.016.10896145
ACGGACA46500.015.616195145
GGGGGGT54400.015.255317145
CGCGTAT9153.4742698E-1015.11638145
TGGGGGG97700.014.511028145
GCGGGGG73000.014.210431145
GTACTGC50800.014.0040071
GAATACC55100.013.5431511
AAGGGGG98950.013.2793455145
GTACTGA57000.013.1789891
GTACTAG22850.013.0630651
CGTCTTA18550.001731738313.0485735145
ATGCCGT98350.013.00877145
TGCCGTA22254.7895734E-412.432797145
GTACTAC28650.012.1549581
Produced by FastQC (version 0.11.5)