FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004996173

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004996173
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15154539
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGTCTA99550.026.09377145
CCTGTCG41950.021.891674145
TGACGCG19859.458745E-1118.505909145
CGCCGTA52100.017.62679145
GTACTAG28200.017.0590021
CGTATCA55800.016.457987145
GTCCTAG52950.016.3121451
GTTCTAG65700.016.0587581
GTGCTAG49150.016.016181
GTACTAA45300.015.8085891
GTATTAG47700.015.2423961
CCGTATA13959.5022835E-515.047303145
GCCGTAT55550.014.642678145
AGGGGGG117150.014.558411145
TAGATCG14651.4415378E-414.32832145
CGAATAT28551.0859367E-913.785675145
GACGAAA37151.8189894E-1213.419541145
GTGCTAA57750.013.1577861
GTATAGG36900.013.036961
GTCCTAC48300.013.0157951
Produced by FastQC (version 0.11.5)