FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004996177

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004996177
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15235373
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGTCTA103850.021.003033145
CCGTATA15954.249341E-818.344532145
CGTATCA55550.017.717087145
TAGATCG14054.723657E-617.03887145
AGGGGGG111650.016.915089145
GTCCTAG55950.016.696271
GCCGTAT56000.016.624733145
GACGAAA36450.016.054611145
CGTATAA18802.7688657E-715.56358145
GTGCTAG52300.015.4590751
GTACTAG27850.015.1038971
GTACTAA44500.014.8541861
GTCCTAA46950.014.7771821
GCTCTAG65850.014.7668441
GTATAGG35150.014.7646031
GTATTAG49450.014.4719981
CCTGTCG44150.014.459563145
GGGGGGG1819100.014.388422145
GTCGCCG74600.014.262506145
CGCCGAA35600.014.1964145
Produced by FastQC (version 0.11.5)