FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004996199

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004996199
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15994758
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTATA15359.094947E-1218.901678145
GTCCTAG60700.018.4214861
GTTCTAG73900.016.6602441
GTACTAG33300.016.6109391
GTACTAA55600.016.046191
GTGCTAG66600.015.0927351
CTGTCTA115750.014.705479145
GTCCTAA54700.014.570451
GGTCTAA51550.013.8454821
GTCTTAG62600.013.6818121
CGTATCA51200.013.600348145
CCTGTCG45550.013.588737145
GTACAGG106400.013.4719631
TGTACCG23500.013.3903365
GTCCTAT57750.013.3889611
GTGCTAA67250.013.1779991
GTATACG11700.012.7089481
CGCCGTA49050.012.6191025145
GCTCTAG74000.012.6189561
CTAGGCT77050.012.5609614
Produced by FastQC (version 0.11.5)