FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004996200

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004996200
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15730640
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGTCTA119200.021.327982145
GGGGGGG660350.021.263409145
GTACTAG32700.015.612651
GTCCTAG61100.014.4767471
GTATAGG43050.014.2033231
GTACAGG107050.014.0855231
GTATTAG58700.014.0573231
GTTCTAG74450.013.7148051
GTACTAA49700.013.6167781
CTAGACT52400.012.9143294
GTGTAGG81250.012.8592381
GTCTTAG60400.012.7771021
GCCCTAG55800.012.7665331
GCGCTAG8855.456968E-1212.7448941
GTGCTAG62400.012.6529861
GTATTAA96350.012.5691051
GTACAGA121400.012.4694541
CTATACT63100.012.4177494
GTATATA112250.012.3752921
TGCACTG168400.012.3374575
Produced by FastQC (version 0.11.5)