FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004996201

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004996201
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15730640
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCTAG63600.018.455661
GTGCTAG60750.017.359911
CCGTATA15704.978574E-915.9590025145
GTTCTAG75850.015.789231
CGCCGTA45300.015.316741145
GTATTAG60400.014.6983921
GTATAGG43250.014.6029391
GTACTAG34750.014.4027361
GTACTAA52450.014.3134891
GTACAGG106650.013.9110191
GTCTAGA62450.013.5480381
CTGTCTA119000.013.442906145
GTCCTAA57550.013.3556451
CCTAGAC56100.013.250683
GCTCTAG70000.013.1082031
CTAGACT54650.012.9440354
GTCCTAT57650.012.8157161
GTCTTAG57700.012.8046091
CTATACT61950.012.7621324
TCTATAC60750.012.7258873
Produced by FastQC (version 0.11.5)