FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004996204

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004996204
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14750157
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGTCTA98500.045.226414145
GGGGGGG625400.040.472294145
CGCGTAT8151.5517998E-420.704597145
AGGGGGG79050.018.78474145
CGGGGGG75900.017.341124145
TATGCCG76350.017.238916145
ATGCCGT90600.016.389997145
CGTCTTA18556.8964364E-616.373934145
GTACTAG27650.014.8520471
GTGCTAG51950.014.7963341
TCGTATG77000.014.463642145
TATCGTA26100.014.223504145
GCGGGGG94950.013.86194145
GTCCTAG49600.013.6928821
GTATAGG33900.013.6668931
GGCGGGG111800.013.5839205145
CGAGACA177300.013.514626145
GGCGTAT15150.008070113.365741145
GTTCTAG58300.013.3653381
GTATTAG43400.013.1014961
Produced by FastQC (version 0.11.5)