FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004996209

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004996209
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15117658
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGTCTA103400.031.081114145
TGACGCG19900.024.469217145
CGAATAT26900.021.722116145
CGCCGTA55900.018.002459145
CCTGTCG44000.017.706816145
TCGCGGT22351.0839358E-817.429527145
AAGGGGG101550.017.262184145
GTCGCCG76000.017.085524145
CGTATCA59200.016.998941145
CGGGGGG133200.016.816158145
GTCCTAG51350.016.7840881
AGGGGGG108700.016.72401145
CGACGAA156250.016.620798145
GGGGGGG2089450.016.515175145
GTATTAG47850.015.1387221
CCGACGA154150.014.951912145
GTTCTAG63050.014.67585851
GTATAGG35100.014.6121861
GTACTAA41750.014.5643791
GCCGTAT58350.014.464866145