FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004996211

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004996211
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16537527
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGTCTA104200.034.309834145
CCGTATA16452.2591848E-923.669643145
AGGGGGG124700.023.276215145
CGCCGTA67100.022.683544145
GACGAAA41400.022.229921145
CGTATCA68350.021.750824145
GCCGTAA21603.274181E-1121.303677145
GGGGGGG2367950.018.027676145
CCTGTCG46650.016.693062145
TGACGCG19256.2329236E-616.54919145
GTCGCTT32859.822543E-1116.162958145
GTTCTAG67450.015.972981
GCCGTAT68800.015.949174145
TCGCCGT77900.015.903603145
TACACAA131100.015.659945145
CGACGAA180800.015.466556145
GTGGTCG103150.015.442167145
GTACTAG29100.015.4116061
CGGGGGG145800.015.294949145
CGTATAA20852.9722287E-915.279227145
Produced by FastQC (version 0.11.5)