FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004996216

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004996216
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16347982
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGGGG725750.043.893467145
CTGTCTA105100.041.684914145
CGCGTAT9500.023.449158145
GTCGTAT23250.020.75965145
CGTCTTA21756.689279E-717.070269145
GTACTAG27700.016.2522681
GCGGGGG101900.015.302935145
TATGCCG72900.015.278944145
AGGGGGG90950.015.104232145
ATGCCGT90750.014.728397145
GACGTAT20351.2315325E-414.595709145
GTCCTAG52950.014.4922691
GTACTAA39300.014.3189511
GTATTAG45600.014.0234941
TATCGTA24850.013.4467145
GTTCTAG64050.013.3385431
GTACAGA111100.013.2153581
ACACATA155050.013.170144145
GTATAGG45100.013.158081
CGAGACG149700.013.144792145
Produced by FastQC (version 0.11.5)