FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004996217

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004996217
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16347982
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTATA15500.027.653368145
CGCCGTA68450.026.613083145
CTGTCTA104000.023.354685145
CGTATCA67800.022.6536145
GCCGTAA19154.838512E-1022.38262145
CGAATAT26650.021.444763145
GGGGGGG1941050.021.272552145
GCCGTAT68500.020.857773145
TCGCCGT78600.020.449772145
CCTGTCG46900.019.039942145
TGACGCG21805.199763E-818.023315145
AGGGGGG118600.017.467943145
GACGAAA37750.017.031544145
GTGGTCG104650.016.724583145
GTACTAA43050.016.1163881
GTGCTAG54700.015.9723431
CGCCGAA38500.015.7719965145
GTACTAG26450.015.7386121
GTCGCCG86750.015.646334145
CGTATAA20859.094947E-1215.418245145
Produced by FastQC (version 0.11.5)