FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004996335

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004996335
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25574920
Sequences flagged as poor quality0
Sequence length26
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGGGG207750.019.2777922
CGGGGTC208750.019.247194
TAAGAGA226200.019.2167071
AGAGCGA111000.019.1972241
GGGATGA154500.019.1601241
CTAATAG392700.019.1487673
TCTAATA393550.019.0977692
GGCCGTC138800.019.0771034
GGGGTCT211050.019.0231115
GCTGCGA192450.019.0206931
CGGAGAG85650.018.9957312
ACGGAGA148050.018.9542351
TCTGAGC163500.018.947314
TCGGGGT213700.018.8949493
ACCAGTA415050.018.8899691
CGGGAAG103200.018.884963
TCTAACG287850.018.8768229
GAATGAA109350.018.8739241
CTAACGT287850.018.87353110
TGTAGGA206850.018.8485985
Produced by FastQC (version 0.11.5)