FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004996338

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004996338
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22583143
Sequences flagged as poor quality0
Sequence length124
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1132180.5013385426466104No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAA860140.3808770107863197No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGACAAAAAAAAAAAAAAAAAA638640.2827950033350097No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA459310.2033862159930529No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG362360.1604559648761025No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN338570.14992155874848775No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC265400.11752128567755162No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAAAAAAAAAAAAAAAAAA238670.10568502355938676No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT1007800.090.993711
AGCAGTG1018050.089.5483862
GTGGTAT1039900.087.660276
GTATCAA1064750.086.0981459
GGTATCA1087000.084.0026258
TGGTATC1122800.081.2389147
CAGTGGT1147850.079.57594
GCAGTGG1291600.070.724453
AGTGGTA1468200.062.2877125
CAACGCA1000150.048.82861712-13
ATCAACG1002950.048.66312810-11
TCAACGC989350.046.25938812-13
TACATGG1057900.045.6698122-23
AGAGTAC1093000.044.61744718-19
GCAGAGT1099750.044.29649416-17
GAGTACA1042150.043.91932320-21
GTACATG1058750.043.1252522-23
CGCAGAG1071750.042.81241216-17
AGTACAT1141850.042.71217720-21
CAGAGTA1110800.041.3507818-19
Produced by FastQC (version 0.11.5)