FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004996347

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004996347
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25252245
Sequences flagged as poor quality0
Sequence length26
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTGAT148500.018.9550789
TCGCTGA149150.018.872328
AAAAGTG126300.018.7476865
AAGTGAA155750.018.746677
GGGACCT122750.018.6864721
GACTAAC148700.018.5468671
GGGACGT84550.018.5216333
TGCCAAA147600.018.4614371
AGCGAAG79350.018.4499533
CTCGAAA111150.018.4406931
AGATGCC139000.018.4168
AACCGCG146750.018.3434241
GCCAAGA92600.018.3368362
AAGGCAG110650.018.3341711
CGCTGGA76150.018.3252121
AGGGATG89300.018.3151741
CGGGACG85150.018.2970262
CAGGGTA147350.018.2613858
TACGGAT127500.018.2570651
ACACCGG154450.018.2514691
Produced by FastQC (version 0.11.5)