FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004996349

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004996349
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21921179
Sequences flagged as poor quality0
Sequence length26
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCATGCG186950.019.0430361
GCGTATA200900.018.8805032
TGACGGC112700.018.873621
AGCGTAT211600.018.8615651
GTATAGC201100.018.856844
TATAGCA201800.018.791435
GATCGCG126900.018.779071
CTCGTAC253450.018.7339821
CAGCGAC89650.018.6954251
GCACGCC218600.018.6540899
AGCGGAT125600.018.645388
GAATGAA111150.018.5969891
TAAGAGA123800.018.5950071
ACGAGAG94450.018.5908629
CACGAGA94600.018.5718278
CTCGAGG96600.018.499461
TAAGACG89950.018.4885032
CGAGAGT95100.018.44289210
GTAACGG135850.018.42388210
AGTGAGG82050.018.4039041
Produced by FastQC (version 0.11.5)