FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004996350

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004996350
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21921179
Sequences flagged as poor quality0
Sequence length124
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1088070.49635560204129536No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAA894930.4082490271166528No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA660790.30143907861890096No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGACAAAAAAAAAAAAAAAAAA499810.22800324745306813No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAAAAAAAAAAAAAAAAAA359720.16409701321265613No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN327500.14939889866325165No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG322500.1471179994470188No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC220490.1005830936374362No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT1189400.095.084171
AGCAGTG1216400.092.359752
GTGGTAT1273950.088.097776
GTATCAA1305950.086.449519
GGTATCA1315950.085.4876258
TGGTATC1344250.083.5615547
CAGTGGT1360950.082.4255144
GCAGTGG1487250.075.445953
AGTGGTA1532700.073.352135
CAACGCA1241050.048.6932512-13
ATCAACG1241900.048.6808410-11
TACATGG1297850.045.94336322-23
TCAACGC1230250.045.88949612-13
AGAGTAC1340250.044.9947318-19
GCAGAGT1341700.044.88638316-17
GAGTACA1282650.043.98289520-21
AGTACAT1376300.043.79442220-21
GTACATG1291200.043.50845322-23
CGCAGAG1305800.043.2494916-17
CAGAGTA1357350.041.65019218-19
Produced by FastQC (version 0.11.5)