FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004996355

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004996355
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24544755
Sequences flagged as poor quality0
Sequence length26
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCATGCG206500.019.037881
GTATAGC230500.018.9592914
TATAGCA231200.018.8872185
TGACGGC120250.018.8753721
GATCGCG137800.018.8742711
GCGTATA232000.018.8495642
AGCGTAT244350.018.811321
CTCGAGG110900.018.7799871
GCACGCC251400.018.7538689
CTCGTAC293000.018.7435071
TAAGAGA140900.018.6862011
CAGCGAC100850.018.647821
TAAGACG105250.018.632252
ACGAGAG110000.018.6079969
AGCGGAT142500.018.5671398
GAATGAA130500.018.5579661
TGGGTGA109500.018.4919855
GTAACGG149600.018.4748110
CACGAGA111100.018.432578
ATCAGAC133400.018.4112112
Produced by FastQC (version 0.11.5)