FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004996361

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004996361
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44130327
Sequences flagged as poor quality0
Sequence length26
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCACGGA473100.019.3614431
TGAGAGG416350.019.3104631
TCGTGAA418600.019.292126
GTGGGAG406850.019.2863372
AGAGCGA306800.019.2794481
GAGAGGC358450.019.2319412
GAGCGAC233450.019.2195222
CACGGAG451150.019.2122552
GGGAATG374850.019.151451
GGCGATA363850.019.09805710
TCCGCAG423900.019.0886124
TTGCGTC525450.019.0843811
TTGCAGA437500.019.0712055
AGGCGAT364650.019.0669129
TCCTGTA655750.019.0430498
CAGGCGA366000.019.001948
AGCTTGA381000.018.999791
CCCGACA365050.018.99418810
TGCTACC366900.018.9940641
ACAGGCG362900.018.9934657
Produced by FastQC (version 0.11.5)