FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004996385

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004996385
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26758398
Sequences flagged as poor quality0
Sequence length26
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTGTCTTCCAGTTTTCCACTTCAAA418750.15649292607128423No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGGAG109750.018.860943
CCGGAGA88400.018.8006954
ATGGGAG157300.018.7982651
GGGAGAT96400.018.7924521
AGGGTGA97250.018.7619191
CGGAGAC121000.018.7366582
CGCTGGA141100.018.6949861
GGGATGA96800.018.611461
ACTGAAC121500.018.5986751
TGACGGC108450.018.5768451
TATGCCC194450.018.5403921
CTGGTAG241300.018.5038343
GACTAAC113700.018.5020921
GCTGGAG121450.018.4943122
GGCGTGT79350.018.455941
AGAGTGA98000.018.4468778
CTCAGAC174000.018.4291746
CTGGAGT122800.018.387523
TGCTACC170350.018.3799461
AGGCAAA104300.018.3775798
Produced by FastQC (version 0.11.5)