FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004996387

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004996387
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26455614
Sequences flagged as poor quality0
Sequence length26
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTGTCTTCCAGTTTTCCACTTCAAA405500.15327559587163617No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGGAG111600.018.8892143
CGGAGAC121250.018.8211562
GGGATGA96950.018.7395271
CGCTGGA137350.018.69041
ATGGGAG159050.018.681571
GGGAGAT95200.018.674161
ACTGAAC121800.018.6370771
CCGGAGA94250.018.599794
CGCAGGC101450.018.5102569
GCTGGAG118600.018.4828222
CTGGTAG235550.018.4719813
ACTGAGT136050.018.464551
CTCAGAC176450.018.4474356
AGGGATG123700.018.4316921
TCAGCGT191150.018.4255337
AGAGTGA95200.018.4246188
TGACGGC105800.018.4012681
TATGCCC188100.018.3812711
TGCTACC170200.018.3688131
AGGGTGA96000.018.3622231
Produced by FastQC (version 0.11.5)