FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004996389

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004996389
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29941079
Sequences flagged as poor quality0
Sequence length26
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTGTCTTCCAGTTTTCCACTTCAAA477320.15941977241367955No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGAGAT105800.018.8015061
ATGGGAG181450.018.7768211
CGGAGAC136550.018.764272
GGGATGA111100.018.7331681
CGCTGGA155050.018.6697541
AGAGTGA107750.018.661398
ACTGAAC136950.018.6384741
GGCGACT85200.018.6262551
CCGGAGA97300.018.5923234
ACTGAGT154050.018.5766011
ACCGGAG120150.018.5687963
AGGGTGA109400.018.5669541
CTGGTAG272250.018.5347773
GCTGGAG134050.018.5322882
CTCAGAC191050.018.5062966
AGGCAAA118950.018.4677858
TGACGGC122000.018.4455471
TCAGCGT206400.018.4426127
GTGAAGG112600.018.439181
GAGGGAC67450.018.4155162
Produced by FastQC (version 0.11.5)