FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004996391

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004996391
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23791587
Sequences flagged as poor quality0
Sequence length26
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTGTCTTCCAGTTTTCCACTTCAAA458470.19270257171158864No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGAAAGC177050.019.4107381
AAAGCTC136200.018.8185443
GCGAAGC71800.018.802624
AGACTAG104250.018.722768
CGTGCAG119650.018.6948957
GATGAAA92550.018.6854421
TCGGCGC151800.018.6810198
TGACGGC115400.018.6778221
GTAACTG222350.018.6409241
ATGCGAT113850.018.624631
CGGCGCA91800.018.6037459
GAGCAGA85250.018.5960041
GAAAGCT138300.018.5404952
GCGAGAC46150.018.5268145
ATCCGAA122150.018.5218321
AACTGCA142100.018.5087133
CCCGAAA95050.018.50417910
ACTGCAA142050.018.5009144
GAGCGAA73150.018.4836562
AGAGACA89900.018.3988343
Produced by FastQC (version 0.11.5)