FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004996397

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004996397
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27347894
Sequences flagged as poor quality0
Sequence length26
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTGTCTTCCAGTTTTCCACTTCAAA536330.19611382141527972No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGAAAGC203000.019.3169361
TGACGGC139000.018.9465481
AGACTAG121500.018.8289018
GAGCAGA95750.018.705671
GATGAAA107000.018.6746851
CGTGCAG137900.018.6633477
AAAGCTC157800.018.6566313
CGGCGCA102500.018.6516139
TCGGCGC174000.018.6298358
GTAACTG257800.018.6224291
GCGAAGC84150.018.609734
ATGCGAT133150.018.5991481
AACTGCA163500.018.5444473
CCCGAAA110850.018.53707510
AGAGACA104250.018.5324763
GTGACTA128250.018.5083878
GAGCGAA84900.018.5056112
ACTGCAA163700.018.5034644
GCAAGGG130550.018.4503737
AAAGAGA91650.018.439824
Produced by FastQC (version 0.11.5)