FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004996401

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004996401
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30135048
Sequences flagged as poor quality0
Sequence length26
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTGTCTTCCAGTTTTCCACTTCAAA603200.20016560119632132No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGATGA289000.019.4056971
CGCACTC422900.019.1607239
CAGATGG331300.019.125238
AGATGGC315000.019.0644789
GGTGACC163500.018.9947629
GGCGACT146250.018.9853481
ACCATAG315850.018.98211310
AAACCTG245700.018.9405541
GACTGCG140650.018.8164371
GGACAGA130250.018.8055111
AGCTCTC282250.018.7885551
GAAGTGG150950.018.7789926
CGTGAGA104050.018.76826110
TAAGAGA182500.018.7670271
GAGGGCA116350.018.7455333
AGGGTGA173500.018.719771
ACTGATG150800.018.6712881
ACTGTCC253450.018.6614251
TGAGGGC117200.018.6448862
GATCGCG123000.018.6205351
Produced by FastQC (version 0.11.5)