FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004996403

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004996403
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26674487
Sequences flagged as poor quality0
Sequence length26
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTGTCTTCCAGTTTTCCACTTCAAA510660.191441357428917No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGATGA251850.019.4114721
CGCACTC377650.019.1699739
CAGATGG297750.019.1308678
AGATGGC283400.019.0390179
ACCATAG281150.018.9602410
GGCGACT132500.018.9200951
GGTGACC143550.018.8702759
AGCTCTC255350.018.8359151
ACTGTCC225200.018.803471
GGACAGA115900.018.7816411
TGAGGGC101650.018.7804152
GAAGTGG134500.018.7739436
GACTGCG120850.018.77391
ACTGATG138650.018.7374361
AAACCTG219250.018.716051
ACAGGCG106100.018.69022
TGCCAAA177800.018.6738381
TAAGAGA162600.018.6722351
AGGGTGA152650.018.6704061
TGAAGCG116400.018.6269365
Produced by FastQC (version 0.11.5)